Response to Hofmann et al

نویسندگان

  • E. A. Turley
  • L. Pilarski
  • J. I. Nagy
چکیده

same RHAMM1v4 isoform expressed in the same cells RHAMM belongs to a group of hyaladherins that reguwas later reported to be located exclusively in the cytolate cell motility and cell cycle and that are expressed plasm (Zhang et al., 1998). in the cytoplasm, nucleus, and cell surface, in spite of Questions about the physiological significance of the a lack of signal peptide or transmembrane domain. ComRHAMM transformation data are raised by the fact that plexity of RHAMM distribution and transient expression the link between surface hyaluronan binding and transof its small isoforms lies at the heart of the current formation has become doubtful and that the transformacontroversy. We first reported the full-length long form tion studies used a truncated and incompletely proof the human RHAMM cDNA (Wang et al., 1996). This cessed RHAMM cDNA clone. The same is true of other cDNA was later recloned by Assmann et al. (1998). Hofwork using these truncated murine RHAMM constructs mann et al. (1998) obtained the murine homolog. A (e.g., Zhang et al., 1998). Nonetheless the role of this smaller RHAMM isoform (v4; Hall et al., 1995) was variprotein in transformation still warrants further investigaably detected in 3T3 cells with 59 RACE, primer extention and could yet represent a novel pathway of oncosion, and RT-PCR and corresponded to a minor 70–73 genic activity. kDa protein (references in Zhang et al., 1998). This iso-

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عنوان ژورنال:
  • Cell

دوره 95  شماره 

صفحات  -

تاریخ انتشار 1998